DEVELOPMENT OF ROUTINE RT-PCR ELOSA TESTS FOR FRUIT TREE CERTIFICATION
نویسندگان
چکیده
منابع مشابه
RT-PCR-ELOSA tests on pooled sample units for the detection of virus Y in potato tubers.
A highly sensitive RT-PCR protocol able to detect potato virus Y (PVY) in pooled sample units (tubers) was developed. PVY-specific primers selected in the coat protein gene were found to amplify a 359 bp fragment from diluted crude extract of infected tubers. For the detection of the amplification products, a colorimetric detection procedure in microtiter plates was established. The amplicons a...
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Something I notice often in supervising new graduate students is their frustration with the irreproducibility of their biological data. For example, I am asked frequently ‘‘how many biological replicates do I need for qRT-PCR’’? The answer, of course, lies first with the students themselves. They need as many replicates as will persuade them of the validity of their observations. However, for m...
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ژورنال
عنوان ژورنال: Acta Horticulturae
سال: 2001
ISSN: 0567-7572,2406-6168
DOI: 10.17660/actahortic.2001.550.3